Pervasive transcription constitutes a new level of eukaryotic genome regulation

Pervasive transcription constitutes a new level of eukaryotic genome regulation.

During the past few years, it has become increasingly evident that the expression of eukaryotic genomes is far more complex than had been previously noted. The idea that the transcriptome is derived exclusively from protein-coding genes and some specific non-coding RNAs--such as snRNAs, snoRNAs, tRNAs or rRNAs--has been swept away by numerous studies indicating that RNA polymerase II can be fou...

Regulation of the initiation of eukaryotic transcription.

DNA sequences that determine transcriptional regulation of a typical eukaryotic gene consist of a core promoter, which serves as a binding site for the GTF TFIID, and regulatory promoter or enhancer sequences, which bind transcriptional activators. The RNA polymerase II transcription machinery consists of over 50 proteins which are thought to bind to the core promoter in as few as two steps: bi...

Pervasive Transcription of a Herpesvirus Genome Generates Functionally Important RNAs

Pervasive transcription is observed in a wide range of organisms, including humans, mice, and viruses, but the functional significance of the resulting transcripts remains uncertain. Current genetic approaches are often limited by their emphasis on protein-coding open reading frames (ORFs). We previously identified extensive pervasive transcription from the murine gammaherpesvirus 68 (MHV68) ge...

Review Reverse Transcription in the Eukaryotic Genome :

Combinatorial gene regulation by eukaryotic transcription factors.

Recent structure determinations of high order complexes of eukaryotic transcription factors bound to DNA have revealed that residues from their DNA-binding domains are involved in protein-protein interactions between distinct factors. Protein-protein interactions between transactivation domains and coactivators have also been characterized in a number of recently determined structures. These st...